search  
 


Home

Who are we?

Why
register?


Signup for
registration


Online registration

Log in to register
your trial


Search a trial

NRT en CCMO

Contact

NEDERLANDS





MetaRegister
van CCT (UK)


ISRCTN-Register
van CCT (UK)


X chromosome inactivation in human embryos obtained after either IVF or IVF/ICSI


- candidate number3241
- NTR NumberNTR1299
- ISRCTNISRCTN wordt niet meer aangevraagd.
- Date ISRCTN created
- date ISRCTN requested
- Date Registered NTR25-apr-2008
- Secondary IDsP05.1655C CCMO
- Public TitleX chromosome inactivation in human embryos obtained after either IVF or IVF/ICSI
- Scientific TitleX chromosome inactivation in human embryos obatined after either IVF or IVF/ICSI
- ACRONYMN/A
- hypothesisThe ICSI procedure interferes with the process of X chromosome inactivation in human embryos
- Healt Condition(s) or Problem(s) studiedIn vitro fertilization (IVF), Intracytoplasmic sperm injection (ICSI), X chromosome inactivation
- Inclusion criteria1. Human surplus embryos, not suitable for transfer or cryopreservation, originating from an IVF or ICSI treatment
2. Informed consent from patients
- Exclusion criteria1. Abnormal fertilized embryos
2. Presence of multinucleated blastomeres
- mec approval receivedyes
- multicenter trialno
- randomisedno
- group[default]
- Type[default]
- Studytypeintervention
- planned startdate 1-mei-2008
- planned closingdate1-mei-2010
- Target number of participants420
- InterventionsThe intervention on the embryos is intracytoplasmic sperm injection (ICSI), which is compared with conventional IVF. ICSI is a regular intervention used in case of male subfertility, where one spermatozoon is injected into an oocyte, while in IVF the spermatozoa and the oocyte are brought together within a culture dish.
- Primary outcomeX chromosome inactivation pattern in human surplus embryos
- Secondary outcomeComparison of time pattern of X chromosome inactivation between IVF and ICSI embryos
- TimepointsHuman surplus embryos at several stages of development (ranging between 8-cell and blastocyst stage) will be collected and analysed for the X chromosome inactivation status.
- Trial web siteN/A
- statusopen: patient inclusion
- CONTACT FOR PUBLIC QUERIES A. Montfoort, van
- CONTACT for SCIENTIFIC QUERIESDr. J. Dumoulin
- Sponsor/Initiator University Hospital Maastricht (AZM)
- Funding
(Source(s) of Monetary or Material Support)
University Hospital Maastricht (AZM)
- PublicationsN/A
- Brief summaryThere is concern that in-vitro culture of embryos and/or the ICSI technique might have an adverse effect on embryonic development via epigenetic alterations. In a previous study from our group using blastocysts resulting from surplus embryos obtained after either standard IVF or IVF/ICSI we found a clear sex related growth difference in human blastocysts originating from IVF/ICSI, but not in blastocysts originating from standard IVF. It is as yet unknown which mechanism is responsible for our findings. In in vitro produced female bovine blastocysts it has been shown that both X chromosomes were active while dosage compensation by X-chromosome inactivation did occur in in vivo generated embryos. Therefore, we hypothesize 1) that the process of X-inactivation has a time pattern in human preimplantation embryos comparable with that of the already investigated animal species, and 2) that the ICSI procedure might interfere with the process of X-inactivation in human embryos.
- Main changes (audit trail)
- RECORD25-apr-2008 - 17-mei-2008


  • Indien u gegevens wilt toevoegen of veranderen, kunt u een mail sturen naar nederlands@trialregister.nl