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The effect of cold acclimation on brown adipose tissue.


- candidate number11241
- NTR NumberNTR3341
- ISRCTNISRCTN wordt niet meer aangevraagd.
- Date ISRCTN created
- date ISRCTN requested
- Date Registered NTR28-feb-2012
- Secondary IDs11-3-052 / NL37639.068.11; METC / CCMO
- Public TitleThe effect of cold acclimation on brown adipose tissue.
- Scientific TitleThe effect of cold acclimation on human brown adipose tissue and skeletal muscle mitochondrial uncoupling.
- ACRONYM
- hypothesisChronic cold exposure will increase:
1. Basal metabolism and facultative thermogenesis;
2. Brown adipose tissue activity and volume;
3. Skeletal muscle mitochondrial uncoupling and result in BRITE cell recruitment.
- Healt Condition(s) or Problem(s) studiedBrown adipose tissue, Cold acclimation, Skeletal muscle, Non-shivering, Thermogenesis
- Inclusion criteria1. BMI 18-25;
2. Age 18-30;
3. Females: On oral contraceptive;
4. Caucasians.
- Exclusion criteria1. Diabetes Mellitus;
2. Females: Pregnancy;
3. Participate in physical activity more than 2x/week;
4. Use of Beta-blockers;
5. Cardiovascular diseases;
6. Asthma or other obstructive pulmonary diseases;
7. Elevated fasting blood glucose level (> 5.6 mmol/L);
8. Participation in earlier research that included a PET/CT-scan;
9. Radiation therapy due to medical treatment.
- mec approval receivedyes
- multicenter trialno
- randomisedno
- groupParallel
- TypeSingle arm
- Studytypeintervention
- planned startdate 1-dec-2011
- planned closingdate1-dec-2013
- Target number of participants20
- InterventionsThe volunteers will undergo two PET/CT-scans, in which cold-induced BAT activity will be measured before and after a cold acclimation period of 10 days. To investigate the role of the skeletal muscle mitochondrial uncoupling and BRITE cell recruitment a muscle and fat biopsy sample will be taken prior to the acclimation period and afterwards. Finally, body composition will be determined with a DXA-scan, and skin perfusion and relevant body temperatures will be measured as well.
Cold acclimation is achieved by exposure to an environmental temperature of 16 degrees (cold room) for 10 consecutive days. The first day the subjects will remain in this cold room for 2 hours, the second day for 4 hours and days 3-10 for 6 consecutive hours. The subjects are dressed in t-shirt, shorts and flip flops. For these 6 hours, the subjects are instructed to refrain from physical activity as much as possible. Watching TV and working on a computer are activities that can be done.
- Primary outcome1. Standard uptake values (SUVs) of FDG of active brown adipose tissue with the PET/CT-scans, one time prior and one time post the cold acclimation period (2x 2hour);
2. Energy expenditure with indirect calorimetry, one time prior, half way and one time post the cold acclimation period (3x 3hour);
3. Skeletal muscle mitochondrial uncoupling with the muscle biopsy, one time before and one time post the cold acclimation period (2x 30minutes). BRITE cell recruitment with the fat biopsy, one time prior and one time post the cold acclimation period (2x 30minutes).
- Secondary outcome1. Body temperatures (core and skin temperatures) with iButtons and a telemetric pill, one time prior, half way and one time post the cold acclimation period;
2. Skin perfusion with laser Doppler flowmetry, one time prior, half way and one time post the cold acclimation period;
3. Blood parameters, among others insulin, glucose, free fatty acids and thyroid hormones, one time prior, half way and post the cold acclimation period;
4. Body composition with a DXA-scan, one time prior and post the cold acclimation period.
- TimepointsParticipation will take approximately 70 hours.
- Trial web siteN/A
- statusstopped: trial finished
- CONTACT FOR PUBLIC QUERIESMSc. Anouk Lans, van der
- CONTACT for SCIENTIFIC QUERIESPhD. Wouter Marken Lichtenbelt, van
- Sponsor/Initiator Maastricht University Medical Center (MUMC+)
- Funding
(Source(s) of Monetary or Material Support)
NWO-TOP grant
- PublicationsN/A
- Brief summaryThe volunteers will undergo two PET/CT-scans, in which cold-induced BAT activity will be measured before and after a cold acclimation period of 10 days. To investigate the role of the skeletal muscle mitochondrial uncoupling and BRITE cell recruitment a muscle and fat biopsy sample will be taken prior to the acclimation period and afterwards. Finally, body composition will be determined with a DXA-scan, and skin perfusion and relevant body temperatures will be measured as well.
- Main changes (audit trail)
- RECORD28-feb-2012 - 13-dec-2013


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